Comment on the validity of your estimate. Assuming that the beetles have a non-overlapping generation time of one year, and that the pond represents a closed population (no immigration or emigration), estimate the effective population size of the beetles from the data given below. Allelic diversity was assessed on each occasion at a single polymorphic microsatellite locus. A population of great diving beetles in a large pond was sampled on two separate occasions (20 in the first sampling, 30 in the second) five years apart. Suggest reasons for any differences you find between the populations.ģ. (c) Whether the loci complied with HW expectations in each population. (b) Overall average observed and expected heterozygosities, across all three loci, for each population (a) Observed and expected heterozygosities at each locus Allozyme analysis of 20 individuals from each of two Sitka spruce tree populations, the first in a large forest and the second in a small, isolated woodland yielded the results shown below for three bi-allelic loci: Locus and genotype Outline how the use of neutral molecular markers such as microsatellites can demonstrate differences between these structures.Ģ. Describe the major types of population structure that occur in nature. Rowe, Sweet & Beebee: An Introduction to Molecular Ecology 3e Chapter 7: Questionsġ.
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